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Description

Microbial mats in the submerged Middle Island Sinkhole of Lake Huron provide opportunities to investigate cyanobacteria under such persistent low-O2 Authors: Voorhies, A.A., B.A

10.3390/ijms18122764 86 GuX.ZhouJ.ZhouY.WangH.SiN.RenW.et al (2021)

After centrifugation at 12700 rpm for 25 min at 4 C, 60 l of the supernatant was neutralized with 100 l of 0.25 M CHES-NaOH (pH 9.4), and disulfides were reduced with 35 l of freshly prepared 10 mM dithiothreitol